Mouse TCK-1 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse CXCL7 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of CXCL7/TCK-1 in mouse serum, plasma and cell culture media. An antibody specific for mouse CXCL7/TCK-1 was coated on a 96-well plate. Standards and samples are added to the wells and any CXCL7/TCK-1 present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse TCK-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of CXCL7/TCK-1 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; Inflammation;

specific against

Mouse

detection target

pro-platelet basic protein (TCK-1)
Background: Neutrophil activating peptide 2 (NAP-2), connective tissue activating protein III (CTAP-III) and beta-thrombogulin (beta-TG), are proteolytically processed carboxy-terminal fragments of platelet basic protein (PBP), which is found in the alpha-granules of human platelets. NAP-2 is a member of the CXC chemokines and is named CXCL7. Similar to other ELR domain containing CXC chemokines, such as IL-8 and the GRO proteins, NAP-2 binds CXCR2 and chemoattracts and activates neutrophils. It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator.

alias

2400003M24Rik, AI854500, CTAP3, CTAPIII, Cxcl7, LA-PF4, LDGF, MDGF, NAP-2, NAP-2-L1, Scyb7, TGB, TGB1, THBGB1, b-TG1, beta-TG

sensitivity

10 pg/ml

detection range

10.9-8000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q9EQI5