Mouse TCA-3 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse TCA-3 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of TCA-3 in mouse serum, plasma and cell culture media. An antibody specific for mouse TCA-3 was coated on a 96-well plate. Standards and samples are added to the wells and any TCA-3 present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse TCA-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of TCA-3 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; Inflammation;

specific against

Mouse

detection target

chemokine (C-C motif) ligand 1 (TCA-3)
Background: I-309, a member of the CC subfamily of chemokines, was initially identified by subtractive hybridization as a transcript that was present in a gamma/delta T cell line, but not in EBV-transformed B cells. Human I-309 is assumed to be a homolog of the mouse TCA-3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8.

alias

BF534335, I-309, Scya1,Tca-3

sensitivity

15 pg/ml

detection range

32.8-8000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P10146