Mouse Semaphorin 4D / SEMA4D / CD100 ELISA Pair Set

Brand

Sino Biological

short description

The Mouse Semaphorin 4D / SEMA4D / CD100 ELISA Pair Set is for the quantitative determination of Mouse Semaphorin 4D / SEMA4D / CD100. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Semaphorin 4D / SEMA4D / CD100 coated on a 96-well plate. Standards and samples are added to the wells, and any Semaphorin 4D / SEMA4D / CD100 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Semaphorin 4D / SEMA4D / CD100 monoclonal antibody is then added, producing an antibody-antigen-antibody ﾓsandwichﾔ. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Semaphorin 4D / SEMA4D / CD100 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

spezie

Mouse

detection target

SEMA4D
Standard (antigene): Each vial contains 150 ng of recombinant mouse SEMA4D. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/mL is recommended.
capture antibody type: 1.0 mg/mL of rabbit anti-mouse SEMA4D monoclonal antibody. Dilute to a working concentration of 2 µg/mL in CBS before coating. (Catalog: # 50642-R121)
detection antibody type: 0.5 mg/mL rabbit anti-mouse SEMA4D monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 µg/mL in detection antibody dilution buffer before use. (Catalog: # 50642-R006)
Lable: HRP

sensitivity

The minimum detectable dose of Mouse Semaphorin 4D / SEMA4D / CD100 was determined to be approximately 47 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Detection limitation

47 pg/ml

certificate

ISO9001-2008

pack size

5 and 15 Plates

storage and Stability

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.