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Mouse SDF-1 alpha ELISA Kit

Product Code: BAS-3020136

Verfügbarkeit: Auf Lager

Mouse SDF-1 alpha ELISA Kit

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Beschreibung

Details

Mouse SDF-1 alpha ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse SDF-1 alpha ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of SDF-1 alpha in mouse serum, plasma and cell culture media. An antibody specific for mouse SDF-1 alpha was coated on a 96-well plate. Standards and samples are added to the wells and any SDF-1 alpha present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse SDF-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of SDF-1 alpha present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; Inflammation;

specific against

Mouse

detection target

chemokine (C-X-C motif) ligand 12( SDF-1 α )
Background: CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. CXCL12 is expressed in many tissues in mice including brain, thymus, heart, lung, liver, kidney, spleen, and bone marrow. CXCL12 is strongly chemotactic for lymphocytes. During embryogenesis, it directs the migration of hematopoietic cells from fetal liver to bone marrow and the formation of large blood vessels. It has also been shown that CXCL12 signalling regulates the expression of CD20 on B cells. CXCL12 is also chemotactic for mesenchymal stem cells and is expressed in the area of inflammatory bone destruction, where it mediates their suppressive effect on osteoclastogenesis.

alias

Pbsf, Scyb12, Sdf1, Tlsf, Tpar1

sensitivity

2 pg/ml

detection range

9.4-600 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P40224

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller BioAim Scientific
Herstellerland Kanada
Sensitivity 2 pg/ml
Specific Against Mouse
Antigen Name chemokine (C-X-C motif) ligand 12
Detection Range 9.4-600 pg/ml
Alias Pbsf, Scyb12, Sdf1, Tlsf, Tpar1
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