BioAim Scientific (Kanada)
The Bioaim Mouse SDF-1 alpha ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of SDF-1 alpha in mouse serum, plasma and cell culture media. An antibody specific for mouse SDF-1 alpha was coated on a 96-well plate. Standards and samples are added to the wells and any SDF-1 alpha present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse SDF-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of SDF-1 alpha present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Angiogenesis; Inflammation;
chemokine (C-X-C motif) ligand 12( SDF-1 α ) Background: CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. CXCL12 is expressed in many tissues in mice including brain, thymus, heart, lung, liver, kidney, spleen, and bone marrow. CXCL12 is strongly chemotactic for lymphocytes. During embryogenesis, it directs the migration of hematopoietic cells from fetal liver to bone marrow and the formation of large blood vessels. It has also been shown that CXCL12 signalling regulates the expression of CD20 on B cells. CXCL12 is also chemotactic for mesenchymal stem cells and is expressed in the area of inflammatory bone destruction, where it mediates their suppressive effect on osteoclastogenesis.
Pbsf, Scyb12, Sdf1, Tlsf, Tpar1
Serum, plasma, Cell culture supernatant
|Antigen Name||chemokine (C-X-C motif) ligand 12|
|Detection Range||9.4-600 pg/ml|
|Alias||Pbsf, Scyb12, Sdf1, Tlsf, Tpar1|