Mouse Renin 1 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse Renin 1 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Renin 1 in mouse serum, plasma and cell culture media. An antibody specific for mouse Renin 1 was coated on a 96-well plate. Standards and samples are added to the wells and any Renin 1 present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse Renin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Renin 1 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Peptide hormone;

specific against

Mouse

detection target

renin 1 structural( Renin 1 )
Background: Renin is a secreted endopeptidase that plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of Renin. The 406 aa precursor includes a signal peptide (aa 1 – 23), a propeptide (aa 24 – 66), and a mature chain (aa 67 – 406). The amino acid sequence of human Renin is 100%, 73%, 71% and 67% identical to that of chimpanzee, canine, mouse and rat. Mouse Renin is a secreted, 42 – 47 kDa glycosylated member of the peptidase A1 family. It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I. In mouse, there are two genes that code for Renin. One is in the submandibular gland and the other is in the kidney. The two mature Renin molecules are 95% amino acid (aa) identical. Renal Renin (Renin-1) is synthesized as a 381 aa proform (aa 22 – 402).

alias

D19352, Ren, Ren-1, Ren-Ac, Ren1d, Rn-1, Rnr, Ren1

sensitivity

5 pg/ml

detection range

12.2-50000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P06281