BioAim Scientific (Kanada)
The Bioaim Mouse Progranulin ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Progranulin in mouse serum, plasma and cell culture media. An antibody specific for mouse Progranulin was coated on a 96-well plate. Standards and samples are added to the wells and any Progranulin present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse Progranulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Progranulin present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Cancer Biomarker;
granulin(Progranulin) Background: Progranulin, also known as proepithelin and PC cell-derived growth factor (PCDGF), is a secreted, glycosylated protein with multiple repeats of the granulin/epithelin motif. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and prostate cancer (PC) cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
Serum, plasma, Cell culture supernatant
|Detection Range||20.6-15000 pg/ml|