BioAim Scientific (Kanada)
The Bioaim Mouse PRDC (protein related to DAN and Cerberus) ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of PRDC in mouse serum, plasma and cell culture media. An antibody specific for mouse PRDC was coated on a 96-well plate. Standards and samples are added to the wells and any PRDC present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse PRDC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of PRDC present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Embryogenesis and morphogenesis;
gremlin 2, DAN family BMP antagonist ( PRDC ) Background: PRDC (protein related to DAN and Cerberus) is a secreted, cysteine knot-containing BMP antagonist belonging to the Cerberus/DAN (CAN) family. Mammalian CAN family members, including Gremlin, DAN, Cerberus, COCO, SOST, and USAG-1, have the conserved 6 cysteine residues that form a cysteine knot, and two additional cysteine residues located in the cysteine knot loops that form an additional intrasubunit disulfide bond. Some members of this family, including PRDC, have an additional cysteine residue used for dimerization.
Serum, plasma, Cell culture supernatant
|Antigen Name||gremlin 2, DAN family BMP antagonist|
|Detection Range||3.3-800 pg/ml|