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Boster
For quantitative detection of mouse PECAM-1 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Mouse
There is no detectable cross-reactivity with other relevant proteins.
Mouse PECAM-1Protein name: Platelet endothelial cell adhesion moleculeGene Full Name: platelet/endothelial cell adhesion molecule 1fabbreviation: PECAM1Background: PECAM-1 is an important target GP in DITP.1 Platelet/endothelial cell adhesion molecule-1(PECAM-1) is a 130-kD member of the Ig gene superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and selective T-cell subsets.2 Platelet endothelial cell adhesion molecule-1(PECAM-1/CD31) is a member of the immunoglobulin(Ig) superfamily that has distinctive features of an immunoreceptor based upon its genomic structure and the presence of intrinsic immunoreceptor tyrosine inhibitory motifs(ITIMs) in its ligand binding polypeptide.3 PECAM-1 has recently been shown to contain functional immunoreceptor tyrosine-based inhibitory motifs(ITIMs) within its cytoplasmic domain, and co-ligation of PECAM-1 with the T-cell antigen receptor(TCR) results in tyrosine phosphorylation of PECAM-1, recruitment of Src homology 2 domain-containing protein tyrosine phosphatase-2(SHP-2), and attenuation of TCR-mediated cellular signaling.4 The standard used in this kit is the product of gene recombinant expression(extracellular part), consisting of 563 amino acids(E28-K590) with the molecular weight of 65.5KDa. After glycosylating, its molecular weight changes to 91-100KDa. ID:Q08481 ID:18613
CD31|CD31 antigen|EndoCAM|GPIIA|HEC7|Leukocyte surface protein|PECA1|PECA1_HUMAN|PECAM 1|PECAM-1|Pecam1|Platelet endothelial cell adhesion molecule|Platelet/endothelial cell adhesion molecule
Nativ and recombinant mouse PECAM-1
a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
NSO, E18-K590
<10pg/ml
156pg/ml-10000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|1425|2256|4926|1614|2648|5830 Standard deviation|89.8|169.2|280.8|111.4|214.5|378.95 CV(%)|6.3|7.5|5.7|6.9|8.1|6.5
15-20
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s mouse PECAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PECAM-1 has been precoated onto 96-well plates. Standards(NSO, E18-K590) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PECAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse PECAM-1 amount of sample captured in plate.
Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse PECAM-1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each mouse PECAM-1 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.032|0.149|0.245|0.413|0.751|1.246|1.952|2.170
Catalog number|Description|Quantity-|96-well plate precoated with anti- mouse PECAM-1 antibody|1ST0000-10|Lyophilized recombinant mouse PECAM-1 standard|10ng/tube�2AR1107|Biotinylated anti- mouse PECAM-1 antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30 mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <10pg/ml |
Specific Against | Mouse |
Antigen Name | Platelet endothelial cell adhesion molecule (PECAM1) |
Detection Range | 156pg/ml-10000pg/ml |
Alias | CD31, CD31 antigen, EndoCAM, GPIIA, HEC7, Leukocyte surface protein, PECA1, PECA1_HUMAN, PECAM 1, PECAM-1, Pecam1, Platelet endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule |