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Mouse PECAM-1/CD31 PicoKine ELISA Kit

Product Code: BOS-EK0874

Verfügbarkeit: Auf Lager

Mouse PECAM-1/CD31 PicoKine ELISA Kit

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Beschreibung

Details

Mouse PECAM-1/CD31 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse PECAM-1 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Mouse PECAM-1
Protein name: Platelet endothelial cell adhesion molecule
Gene Full Name: platelet/endothelial cell adhesion molecule 1f
abbreviation: PECAM1
Background: PECAM-1 is an important target GP in DITP.1 Platelet/endothelial cell adhesion molecule-1(PECAM-1) is a 130-kD member of the Ig gene superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and selective T-cell subsets.2 Platelet endothelial cell adhesion molecule-1(PECAM-1/CD31) is a member of the immunoglobulin(Ig) superfamily that has distinctive features of an immunoreceptor based upon its genomic structure and the presence of intrinsic immunoreceptor tyrosine inhibitory motifs(ITIMs) in its ligand binding polypeptide.3 PECAM-1 has recently been shown to contain functional immunoreceptor tyrosine-based inhibitory motifs(ITIMs) within its cytoplasmic domain, and co-ligation of PECAM-1 with the T-cell antigen receptor(TCR) results in tyrosine phosphorylation of PECAM-1, recruitment of Src homology 2 domain-containing protein tyrosine phosphatase-2(SHP-2), and attenuation of TCR-mediated cellular signaling.4 The standard used in this kit is the product of gene recombinant expression(extracellular part), consisting of 563 amino acids(E28-K590) with the molecular weight of 65.5KDa. After glycosylating, its molecular weight changes to 91-100KDa.
UniProd ID:Q08481
NCBI ID:18613

alias

CD31|CD31 antigen|EndoCAM|GPIIA|HEC7|Leukocyte surface protein|PECA1|PECA1_HUMAN|PECAM 1|PECAM-1|Pecam1|Platelet endothelial cell adhesion molecule|Platelet/endothelial cell adhesion molecule

Origin

Nativ and recombinant mouse PECAM-1

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

NSO, E18-K590

sensitivity

<10pg/ml

detection range

156pg/ml-10000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1425|2256|4926|1614|2648|5830
Standard deviation|89.8|169.2|280.8|111.4|214.5|378.95
CV(%)|6.3|7.5|5.7|6.9|8.1|6.5

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse PECAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PECAM-1 has been precoated onto 96-well plates. Standards(NSO, E18-K590) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PECAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse PECAM-1 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse PECAM-1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each mouse PECAM-1 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.032|0.149|0.245|0.413|0.751|1.246|1.952|2.170

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse PECAM-1 antibody|1
ST0000-10|Lyophilized recombinant mouse PECAM-1 standard|10ng/tube�2
AR1107|Biotinylated anti- mouse PECAM-1 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller Boster
Herstellerland USA
Sensitivity <10pg/ml
Specific Against Mouse
Antigen Name Platelet endothelial cell adhesion molecule (PECAM1)
Detection Range 156pg/ml-10000pg/ml
Alias CD31, CD31 antigen, EndoCAM, GPIIA, HEC7, Leukocyte surface protein, PECA1, PECA1_HUMAN, PECAM 1, PECAM-1, Pecam1, Platelet endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule
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Datasheet