Mouse OPN ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse OPN ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of OPN in mouse serum, plasma and cell culture media. An antibody specific for mouse OPN was coated on a 96-well plate. Standards and samples are added to the wells and any OPN present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse OPN antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of OPN present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; Cancer Biomarker;

specific against

Mouse

detection target

secreted phosphoprotein 1 (OPN)
Background: steopontin (OPN) is also known as bone sialoprotein I (BSP-1 or BNSP), early T-lymphocyte activation (ETA-1), secreted phosphoprotein 1 (SPP1), 2ar and Rickettsia resistance (Ric). The central region of OPN contains RGD and non-RGD binding sites for multiple integrins. Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins. it is masked in full length OPN but is exposed following OPN cleavage by multiple proteases in tumors and sites of tissue injury. OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell adhesion, and migration. Its expression is upregulated during inflammation, obesity, atherosclerosis, cancer, and tissue damage and contributes to the pathophysiology of these conditions.

alias

2AR, Apl-1, BNSP, BSPI, Bsp, ETA-1, Eta, OP, Opn, Opnl, Ric, Spp-1

sensitivity

2 pg/ml

detection range

2.7-2000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P10923