Mouse MPO EasyTestTM ELISA kit

Brand

BioAim Scientific (Kanada)

short description

The BioAim Mouse MPO EasyTestTM ELISA kit can quantitatively measure MPO in Mouse serum or plasma. It is a simple and rapid technology for the quantitation of antigen in a range of sample matrices. The whole process takes less than 1.5 hours with high accuracy and precision. EasyTestTM ELISA is faster and easier to perform than standard format ELISA with less reagent handling and fewer pipetting steps.
Research Area Inflammation; oxidative stress;

specific against

Mouse

detection target

myeloperoxidase(MPO)
Background: Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide dependent formation of hypochlorus acid (HOCl) and other reactive species. Enzymatically active MPO is a disulfide-linked tetramer that contains two heme groups and two copies each of the heavy and light chains. MPO binds Albumin, MMR, Cytokeratin 1 on vascular endothelial cells, HMW Kininogen, and Integrin CD11b/CD18 on neutrophils. These interactions promote MPO clearance, a reduction of nitric oxide and bradykinin levels, reduced vasodilation, and continued neutrophil activation. Neutrophil MPO is stored in cytoplasmic azurophilic granules. Upon cellular activation and degranulation, MPO is delivered into phagosomes where it is required for the killing of phagocytosed bacteria. Activated neutrophils also release granule contents extracellularly. Elevated plasma MPO levels have been associated with a variety of clinical conditions including systemic inflammation, eclampsia, risk of cardiovascular events, vascular endothelial dysfunction, severity of multiple sclerosis, and prospective mortality and oxidative stress during hemodialysis.

alias

mKIAA4033

sensitivity

370 pg/ml

detection range

1.6-400 ng/ml

sample type

Serum

pack size

1x96 Wells

Assay time

1.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P11247