For the quantitative determination of Mouse IL6R / IL-6R / CD126 concentration in serum.
The average concentration of Mouse IL6RA in 10 normal mouse serum is 10.28 +/- 1.22 ng/mL ranging from 7.73 to 12.26 ng/mL.
This assay recognizes both recombinant and natural Mouse IL6RA. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed. Cross-reactivity was observed to be 10% with 50 pg/mL of recombinant Rat IL6RA.
IL6RAntigene: 50280-M08Hcapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||7.82-500 pg/ml|
|Alias||IL6R / IL-6R / CD126|