For the quantitative determination of Mouse IL1A / IL-1A / IL-1F1 concentration in serum and cell culture supernates.
The average concentration of Mouse IL1A in 10 normal mouse serum is 77.89 +/- 55.74 pg/mL ranging from 17.41 to 165.15 pg/mL. Mouse splenocytes (10E6 cells/mL)ﾠwere cultured forﾠ3 daysﾠin RPMI supplemented with 5% fetal bovine serum and stimulated withﾠ5 ug/mL LPS. Aliquots of the cell culture supernates were removed and assayed for levels of natural mouse IL1A, and measured 8.11 pg/mL.
This assay recognizes both recombinant and natural Mouse IL1A. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
IL1AAntigene: 50114-MNAEcapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||6.56-420 pg/ml|
|Alias||IL1A / IL-1A / IL-1F1|