For the quantitative determination of Mouse IL18R1 concentration in cell lysate.
The concentration of Mouse IL18R1 detected in a A549 cell lysate was 2383.46 pg/mL.The A549 cell lysate' total protein concentration was about 6.6 mg/mL.
This assay recognizes both recombinant and natural Mouse IL18R1. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
IL18R1Antigene: 50094-M08Hcapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||18.75-1200 pg/ml|