For the quantitative determination of IL16 concentration in serum and cell culture supernates.
The average concentration of Mouse IL16 in 10 normal mouse serum is 1230.27 +/- 288.92 pg/mL ranging from 834.89 to 1618.19 pg/mL. Mouse splenocytes (10E6 cells/mL) were cultured in RPMI supplemented with 5% fetal bovine serum and stimulated with 5 µg/mL LPS. Aliquots of the cell culture supernates were removed on days 3 and assayed for levels of natural mouse IL16, and measured 3395.12 pg/mL.
This assay recognizes both recombinant and natural Mouse IL16. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.Cross-reactivity was observed to be 0.46% with 50 ng/mL of recombinant human IL16.
IL16Antigene: 51303-M07Ecapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||5.17-350 pg/ml|