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Mouse IL-6 ELISA Kit

Product Code: BAS-3020018

Verfügbarkeit: Auf Lager

Mouse IL-6 ELISA Kit

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Beschreibung

Details

Mouse IL-6 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse IL-6 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of IL-6 in mouse serum, plasma and cell culture media. An antibody specific for mouse IL-6 was coated on a 96-well plate. Standards and samples are added to the wells and any IL-6 present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IL-6 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Cancer Biomarker; inflammation;

specific against

Mouse

detection target

interleukin 6( IL-6 )
Background: IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response, e.g. during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6 also plays a role in fighting infection, as IL-6 has been shown in mice to be required for resistance against bacterium Streptococcus pneumoniae. In addition, osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. IL-6’s role as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10.

alias

Il-6

sensitivity

< 1 pg/ml

detection range

0.82-600 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P08505

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller BioAim Scientific
Herstellerland Kanada
Sensitivity < 1 pg/ml
Specific Against Mouse
Antigen Name interleukin 6
Detection Range 0.82-600 pg/ml
Alias Il-6
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