Mouse IL-5 ELISA Kit

Brand

Cell Sciences

Description

The Cell Sciences Mouse IL-5 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbentassay for the quantitative measurement of mouse IL-5 in serum, plasma and cell culture supernatants. This assay employs anantibody specific for mouse IL-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-5 present in asample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-5 antibody isadded. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells areagain washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. TheStop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

spezie

Mouse

Cross Reaction

Mouse

detection target

Mouse IL-5
Antigen: IL5

alias

EDF; TRF; IL-5; IL5, interleukin-5, T-cell replacing factor, B cell differentiation factor I, B-cell differentiation factor I, eosinophil differentiation factor

sample size

1

pack size

PP

UNSPSC

41116126 and E