Mouse IL-2 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse IL-2 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Mouse IL-2
Protein name: Interleukin-2
Gene Full Name: interleukin 2
abbreviation: IL2
Background: Interleukin-2(IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2(IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2(IL2) is assigned to chromosome 4. Mouse IL-2 contains 149 amino acids in mature form with the molecular mass of 17.2KDa.
ID:P04351
ID:16183

alias

Aldesleukin|IL 2|IL-2|IL2|IL2_HUMAN|Interleukin 2|Interleukin-2|Interleukin2|Involved in regulation of T cell clonal expansion|Lymphokine|T cell growth factor|T-cell growth factor|TCGF

Origin

Nativ and recombinant mouse IL-2

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

E.coli,A21-Q169

sensitivity

<1pg/ml

detection range

15.6pg/ml-1000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|81|215|590|86|193|575
Standard deviation|4.536|11.61|25.37|5.246|13.32|29.9
CV(%)|5.6|5.4|4.3|6.1|6.9|5.2

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse IL-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-2 has been precoated onto 96-well plates. Standards (E.coli,A21-Q169) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-2 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml mouse IL-2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each mouse IL-2 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.067|0.111|0.166|0.288|0.453|1.080|1.669|2.221

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse IL-2 antibody|1
ST0000-10|Lyophilized recombinant mouse IL-2 standard|10ng/tubex2
AR1107|Biotinylated anti- mouse IL-2 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4