Profile Informationen
Login Daten
oder Einloggen
BioAim Scientific (Kanada)
The Bioaim Mouse IL-17B ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of IL-17B in mouse serum, plasma and cell culture media. An antibody specific for mouse IL-17B was coated on a 96-well plate. Standards and samples are added to the wells and any IL-17B present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-17B antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IL-17B present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Inflammation;
Mouse
interleukin 17B (IL-17B) Background: The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.
1110006O16Rik, 1700006N07Rik, Zcyto7
10 pg/ml
16.5-12000 pg/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
Q9QXT6
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | 10 pg/ml |
Specific Against | Mouse |
Antigen Name | interleukin 17B |
Detection Range | 16.5-12000 pg/ml |
Alias | 1110006O16Rik, 1700006N07Rik, Zcyto7 |