×

Registrierung

Profile Informationen

Login Daten

oder Einloggen

Vorname ist erforderlich!
Nachname ist erforderlich!
Vorname ist ungültig!
Nachname ist ungültig!
Dies ist keine E-Mail-Adresse!
E-Mail-Adresse ist erforderlich!
Dies E-Mail-Adresse ist bereit registriert!
Passwort ist erforderlich!
Geben Sie ein gültiges Passwort ein!
Bitte geben Sie 6 oder mehr Zeichen ein!
Bitte geben Sie 16 oder weniger Zeichen ein!
Passwörter sind nicht dasselbe!
Geschäftsbedingungen sind erforderlich!
E-Mail oder Passwort ist falsch!

Mouse IGF-1 ELISA Kit

Product Code: BAS-3020025

Verfügbarkeit: Auf Lager

Mouse IGF-1 ELISA Kit

Bewerten Sie dieses Produkt als Erster

OR

Fragen zur Lieferzeit

Angebot anfordern

Beschreibung

Details

Mouse IGF-1 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse IGF-1 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of IGF-1 in mouse serum, plasma and cell culture media. An antibody specific for mouse IGF-1 was coated on a 96-well plate. Standards and samples are added to the wells and any IGF-1 present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse IGF-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IGF-1 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Cancer Biomarker; Aging;

specific against

Mouse

detection target

insulin-like growth factor 1 ( IGF-1 )
Background: Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Production is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. Approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). IGFBP-3, the most abundant protein, accounts for 80% of all IGF binding. IGF-1 binds to IGFBP-3 in a 1:1 molar ratio. IGFBP-1 is regulated by insulin. IGF-1 binds to at least two cell surface receptors: the IGF-1 receptor (IGF1R), and the insulin receptor. Insulin-like growth factor 1 receptor (IGF-1R) and other tyrosine kinase growth factor receptors signal through multiple pathways. A key pathway is regulated by phosphatidylinositol-3 kinase (PI3K) and its downstream partner, the mammalian target of rapamycin (mTOR). Rapamycins complex with FKBPP12 to inhibit the mTORC1 complex. mTORC2 remains unaffected and responds by upregulating Akt, driving signals through the inhibited mTORC1. Phosphorylation of eukaryotic initiation factor 4e (eif-4E) [4EBP] by mTOR inhibits the capacity of 4EBP to inhibit eif-4E and slow metabolism.

alias

C730016P09Rik,Igf-1, Igf-I

sensitivity

1 pg/ml

detection range

2.7-2000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P05017

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller BioAim Scientific
Herstellerland Kanada
Sensitivity 1 pg/ml
Specific Against Mouse
Antigen Name insulin-like growth factor 1 
Detection Range 2.7-2000 pg/ml
Alias C730016P09Rik,Igf-1, Igf-I
Bewertungen
Tags

Produkttags

Verwenden Sie Leerzeichen um Tags zu trennen. Verwenden Sie Apostrophe (') für Phrasen.

Datasheet

Video