Mouse IFN-gamma EasyTestTM ELISA kit

Brand

BioAim Scientific (Kanada)

short description

The BioAim Mouse IFN-gamma EasyTestTM ELISA kit can quantitatively measure IFN-gamma in mouse serum or plasma. It is a simple and rapid technology for the quantitation of antigen in a range of sample matrices. The whole process takes less than 1.5 hours with accuracy and precision. EasyTestTM ELISA is faster and easier to perform than standard format ELISA with less reagent handling and fewer pipetting steps.
Research Area Inflammation;

specific against

Mouse

detection target

interferon, gamma(IFN-γ)
Background: IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. FNβ is secreted by T helper cells (specifically, Th1 cells), cytotoxic T cells (TC cells) and NK cells only. IFNβ is the only Type II interferon and it is serologically distinct from Type I interferons; it is acid-labile, while the type I variants are acid-stable. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

alias

IFN-g, Ifg

sensitivity

4 pg/ml

detection range

16.4-4000 pg/ml

sample type

Serum

pack size

1x96 Wells

Assay time

1.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P01580