For the quantitative determination of Mouse HER3 / ErbB3 concentration in serum.
The average concentration of mouse KIT detected in 10 mouse serum is 1.94 +/ǂ.58ng/mL ranging from 1.12 to 2.96 ng/mL.
This assay recognizes both recombinant and natural mouse ErbB3. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.A sample containing 50 ng/mL of recombinant human ErbB3 reads as 0.7ng/mL(1.4% cross-reactivity).A sample containing 50 ng/mL of recombinant rat ErbB3 reads as 3.8ng/mL(7.5% cross-reactivity).A sample containing 50 ng/mL of recombinant Cynomolgus ErbB3 reads as 0.1ng/mL(0.2% cross-reactivity).
ERBB3Antigene: 51003-M08Hcapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||39-2500 pg/ml|
|Alias||HER3 / ErbB3|