BioAim Scientific (Kanada)
The Bioaim Mouse GITR L ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of GITRL in mouse serum, plasma and cell culture media. An antibody specific for mouse GITR L was coated on a 96-well plate. Standards and samples are added to the wells and any GITR L present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse GITR L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of GITR L present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Inflammation;
tumor necrosis factor (ligand) superfamily, member 18(GITRL) Background: GITR Ligand, also known as TNFSF18 and TL6, is a transmembrane glycoprotein that is expressed on antigen presenting cells, CD4-CD8- double negative thymic precursors, vascular endothelial cells, neurons, and in the eye. Its expression is transiently upregulated by proinflammatory stimulation. The binding of GITR Ligand to GITR on mouse CD25+ Treg cells permits the reactivation of T cells from Treg-induced suppression. GITR Ligand binding to GITR additionally provides a costimulatory signal to activated CD4+ and CD8+ T cells and NK cells. This interaction also induces reverse signaling in GITR Ligand expressing dendritic cells to suppress cellular activation through the same pathway induced by the immunosuppressant dexamethasone. In the brain, GITR Ligand/GITR interactions enhance NGF-mediated neurite outgrowth from sympathetic neurons. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells.
Serum, plasma, Cell culture supernatant
|Antigen Name||tumor necrosis factor (ligand) superfamily, member 18|
|Detection Range||4.1-1000 pg/ml|