BioAim Scientific (Kanada)
The Bioaim Mouse GAS6 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of GAS6 in mouse serum, plasma and cell culture media. An antibody specific for mouse GAS6 was coated on a 96-well plate. Standards and samples are added to the wells and any GAS6 present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse GAS6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of GAS6 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area apoptosis; cell proliferation;
growth arrest specific 6(GAS6) Background: Gas6 (Growth Arrest Specific 6) is a secreted protein that contains an extensively gamma-carboxylated N-terminal Gla domain and is dependent upon vitamin K for activity. Gas6 signals through the receptors Axl, Dtk/Tyro3, and Mer and is inhibited by shed soluble forms of Axl and Mer. Gas6 protects cells from stress-induced apoptosis and promotes phagocytosis of apoptotic cell debris, promotes platelet activation but inhibits inflammatory cytokine production, and inhibits VEGF-induced angiogenesis, and regulates tumor cell invasiveness.
Serum, plasma, Cell culture supernatant
|Antigen Name||growth arrest specific 6|
|Detection Range||20.5-5000 pg/ml|