Mouse Galectin-7 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse Galectin-7 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Galectin-7 in mouse serum, plasma and cell culture media. An antibody specific for mouse Galectin-7 was coated on a 96-well plate. Standards and samples are added to the wells and any Galectin-7 present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse Galectin-7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Galectin-7 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Cell endocytosis, chaperoning and trafficking ;

specific against

Mouse

detection target

lectin, galactose binding, soluble 7 (Galectin-7)
Background: The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell–cell and cell–matrix interactions. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It is expressed at all stages of epidermal differentiation (i.e., in basal and suprabasal layers). It is moderately repressed by retinoic acid. The protein was found mainly in stratified squamous epithelium. The antigen localized to basal keratinocytes, although it was also found, albeit at lower levels, in the suprabasal layers where it concentrated to areas of cell-to-cell contact. The cellular localization and its striking down-regulation in cultured keratinocytes imply a role in cell–cell and/or cell–matrix interactions necessary for normal growth control.

alias

Galectin-7, Pig1

sensitivity

30 pg/ml

detection range

65.5-16000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

O54974