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BioAim Scientific (Kanada)
The Bioaim Mouse G-CSF ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of G-CSF in mouse serum, plasma and cell culture media. An antibody specific for mouse G-CSF was coated on a 96-well plate. Standards and samples are added to the wells and any G-CSF present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse G-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of G-CSF present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Inflammation;
Mouse
Colony stimulating factor 3 (granulocyte)( G-CSF ) Background: Granulocyte-colony stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream. G-CSF (granulocyte-colony stimulating factor) is a secreted glycoprotein that regulates the proliferation, differentiation, and activation of neutrophilic granulocyte lineage cells. It also enhances M-CSF induced monocyte differentiation and the proliferation of Th2-inducing dendritic cells. G-CSF promotes the development of T cell immune tolerance as well as tissue recovery following myocardial infarction and cerebral ischemia. It signals through G-CSF R/CD114 on monocytes, neutrophils, megakaryocytes, platelets, myeloid progenitors, trophoblasts and placenta, endothelial cells, and various tumor cell types. Mutations in G-CSF R are associated with congenital neutropenia.
MGI-IG, Csfg, G-CSF
< 1 pg/ml
0.21-150 pg/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
P09920
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | < 1 pg/ml |
Specific Against | Mouse |
Antigen Name | Colony stimulating factor 3 (granulocyte) |
Detection Range | 0.21-150 pg/ml |
Alias | MGI-IG, Csfg, G-CSF |