Mouse Fractalkine ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse Fractalkine ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Fractalkine in mouse serum, plasma and cell culture media. An antibody specific for mouse Fractalkine was coated on a 96-well plate. Standards and samples are added to the wells and any Fractalkine present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse Fractalkine antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Fractalkine present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; Cancer Stem Cell Markers; Inflammation;

specific against

Mouse

detection target

chemokine (C-X3-C motif) receptor 1 (Fractalkine)
Background: CX3CL1, also named neurotactin, is a member of the delta chemokine subfamily that contains a novel C-X3-C motif. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1 is expressed in various tissues including heart, brain, lung, kidney, skeletal muscle, and testis. In rat brain, CX3CL1 expression was found to be localized principally to neurons. The expression of CX3CL1 was also reported to be up-regulated on activated endothelial cells. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T‑lymphocytes but not monocytes. CX3CR1, previously named V28 or chemokine beta receptor‑like 1, has been found to be a specific receptor for CX3CL1.

sensitivity

0.15 ng/ml

detection range

0.27-200 ng/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

O35188