BioAim Scientific (Kanada)
The Bioaim Mouse Flt-3L ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Flt-3L in mouse serum, plasma and cell culture media. An antibody specific for mouse Flt-3L was coated on a 96-well plate. Standards and samples are added to the wells and any Flt-3L present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse Flt-3L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Flt-3L present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Cell survival; proliferation; motility;
fms-related tyrosine kinase 3 ligand( Flt-3L ) Background: Flt-3 Ligand, also known as FL, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation. Animal studies also show Flt-3 Ligand to reduce the severity of experimentally induced allergic inflammation. FLT3L is crucial for steady-state plasmacytoid dendritic cell (pDC) and classical dendritic cell (cDC) development. FLT3L and its receptor are involved in the mammalian immune response to malaria.
Serum, plasma, Cell culture supernatant
|Antigen Name||fms-related tyrosine kinase 3 ligand|
|Detection Range||20.5-5000 pg/ml|