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BioAim Scientific (Kanada)
The Bioaim Mouse DPPIV ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of DPPIV in mouse serum, plasma and cell culture media. An antibody specific for mouse DPPIV was coated on a 96-well plate. Standards and samples are added to the wells and any DPPIV present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse DPPIV antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of DPPIV present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Colon Cancer Stem Cell Markers;
Mouse
dipeptidylpeptidase 4(DPPIV) Background:
Cd26, Dpp-4, THAM
0.3 ng/ml
0.41-100 ng/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
P28843
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | 0.3 ng/ml |
Specific Against | Mouse |
Antigen Name | dipeptidylpeptidase 4 |
Detection Range | 0.41-100 ng/ml |
Alias | Cd26, Dpp-4, THAM |