Mouse Dkk-1 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse Dkk-1 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Dkk-1 in mouse serum, plasma and cell culture media. An antibody specific for mouse Dkk-1 was coated on a 96-well plate. Standards and samples are added to the wells and any Dkk-1 present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse Dkk-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Dkk-1 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Cancer Biomarker;

specific against

Mouse

detection target

dickkopf WNT signaling pathway inhibitor 1 (Dkk-1)
Background: Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the Wnt signaling pathway. Dickkopf WNT signaling pathway inhibitor 1 (Dkk1) is a protein-coding gene that acts from the anterior visceral endoderm. The dickkopf protein encoded by DKK1 is an antagonistic inhibitor of the WNT signaling pathway that acts by isolating the LRP6 co-receptor so that it cannot aid in activating the WNT signaling pathway. DKK1 was also demonstrated to antagonize the Wnt/β-catenin pathway via a reduction in β-catenin and an increase in OCT4 expression. This inhibition plays a key role in heart, head and forelimb development during anterior morphogenesis of the embryo.

alias

mdkk-1

sensitivity

20 pg/ml

detection range

24.6-6000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

O54908