Mouse DAN ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse DAN ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of DAN in mouse serum, plasma and cell culture media. An antibody specific for mouse DAN was coated on a 96-well plate. Standards and samples are added to the wells and any DAN present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse DAN antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of DAN present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Embryogenesis and morphogenesis;

specific against

Mouse

detection target

neuroblastoma, suppression of tumorigenicity 1(DAN)
Background: DAN (differential screening-selected gene aberrative in neuroblastoma) was initially identified as a gene whose expression is downregulated in src-transformed rat fibroblasts. Human DAN was isolated from a normal lung cDNA library using mouse DAN as a probe. DAN has now been shown to be a prototypical member of the DAN family of secreted glycoproteins that are putative antagonists for TGF-beta superfamily proteins. DAN family members share a cysteine-rich domain that is structurally related to the cysteine-knot motif found in TGF-beta superfamily ligands. There are at least five mammalian DAN family members including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante and PRDC (protein related to DAN and cereberus).  The DAN family of proteins are thought to act as antagonists by binding TGF-beta family ligands and preventing their interactions with signaling receptor complexes. It is likely the various DAN family members and other TGF-beta BMP antagonists including Noggin, Chordin, Follistatin and TSG can selectively antagonize the activities of different subsets of TGF-beta superfamily ligands.

alias

D4H1S1733E, DAN, Dana, NO3

sensitivity

20 pg/ml

detection range

40.9-10000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q61477