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BioAim Scientific (Kanada)
The Bioaim Mouse CTACK ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of CTACK in mouse serum, plasma and cell culture media. An antibody specific for mouse CTACK was coated on a 96-well plate. Standards and samples are added to the wells and any CTACK present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse CTACK antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of CTACK present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Inflammation;
Mouse
chemokine (C-C motif) ligand 27A (CTACK) Background: CCL27, also known as Cutaneous T cell-attracting chemokine (CTACK), ALP, ILC and ESkine, is a CC chemokine discovered using expressed sequence tags (EST) in a homology search for novel chemokines. It is a small cytokine belonging to the CC chemokine family also known under the names IL-11 R-alpha-locus chemokine (ILC), Skinkine, ESkine and Cutaneous T-cell-attracting chemokine (CTACK). It is associated with homing of memory T lymphocytes to the skin, and plays a role in T cell-mediated inflammation of the skin. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin. It elicits its chemotactic effects by binding to the chemokine receptor CCR10. Mature human and mouse CCL27 are 84% similar. Mouse CCL27 shares 37% and 35% amino acid identity with mouse MIP-3 beta and mouse TECK, respectively.
ALP, AW558992, CTACK, CTAK, Ccl27, ESkine, ILC, PESKY, Scya27, Scya27a
15 pg/ml
24.7-18000 pg/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
Q9Z1X0
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | 15 pg/ml |
Specific Against | Mouse |
Antigen Name | chemokine (C-C motif) ligand 27A |
Detection Range | 24.7-18000 pg/ml |
Alias | ALP, AW558992, CTACK, CTAK, Ccl27, ESkine, ILC, PESKY, Scya27, Scya27a |