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Mouse CD86/B7-2 PicoKine ELISA Kit

Product Code: BOS-EK0711

Verfügbarkeit: Auf Lager

Mouse CD86/B7-2 PicoKine ELISA Kit

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Beschreibung

Details

Mouse CD86/B7-2 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse CD86 in cell culture supernates and serum.

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Mouse CD86
Protein name: T-lymphocyte activation antigen CD86
Gene Full Name: CD86 molecule
abbreviation: CD86
Background: Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.
UniProd ID:P42082
NCBI ID:12524

alias

Activation B7 2 antigen|B7 2|B70|B72|BU63|CD28LG2|CD86 antigen (CD28 antigen ligand 2 B7 2 antigen)|CD86 molecule|CTLA 4 counter receptor B7.2|FUN 1|FUN1|T lymphocyte activation antigen CD86 precursor

Origin

Nativ and recombinant mouse CD86

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

NSO, V26-G245

sensitivity

<10pg/ml

detection range

62.5pg/ml-4000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|0.21|1.44|2.59|0.23|1.47|2.46
Standard deviation|0.011|0.084|0.161|0.013|0.095|0.150
CV(%)|5.3|5.9|6.2|5.8|6.5|6.1

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse CD86 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD86 has been precoated onto 96-well plates. Standards (NSO, V26-G245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD86 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD86 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse CD86 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each mouse CD86 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.067|0.125|0.170|0.307|0.525|0.897|1.449|2.211

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse CD86 antibody|1
ST0000-10|Lyophilized recombinant mouse CD86 standard|10ng/tube�2
AR1107|Biotinylated anti- mouse CD86 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller Boster
Herstellerland USA
Sensitivity <10pg/ml
Specific Against Mouse
Antigen Name T-lymphocyte activation antigen CD86 (CD86)
Detection Range 62.5pg/ml-4000pg/ml
Alias Activation B7 2 antigen, B7 2, B70, B72, BU63, CD28LG2, CD86 antigen (CD28 antigen ligand 2 B7 2 antigen), CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, FUN1, T lymphocyte activation antigen CD86 precursor
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Datasheet