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Mouse CD6 PicoKine ELISA Kit

Product Code: BOS-EK1287

Verfügbarkeit: Auf Lager

Mouse CD6 PicoKine ELISA Kit

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Beschreibung

Details

Mouse CD6 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse CD6 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

mouse CD6
Protein name: T-cell differentiation antigen CD6
Gene Full Name: CD6 molecule
abbreviation: CD6
Background: CD6(Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. What′s more, this gene may be associated with susceptibility to multiple sclerosis.
UniProd ID:Q61003
NCBI ID:12511

alias

CD_antigen=CD6|CD6|CD6 antigen Tp120|CD6 molecule|CD6_HUMAN|FLJ44171|OX52|T cell differentiation antigen CD6|T-cell differentiation antigen CD6|T12|TP120

Origin

Nativ and recombinant mouse CD6

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

NSO, L18-G396

sensitivity

<10pg/ml

detection range

62.5pg/ml-4000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|798|1880|3505|1036|2040|3487
Standard deviation|33.5|67.68|189.27|60|95.88|240.6
CV(%)|4.2|3.6|5.4|5.8|4.7|6.9

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse CD6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD6 has been precoated onto 96-well plates. Standards(NSO, L18-G396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD6 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse CD6 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each mouse CD6 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.014|0.059|0.100|0.185|0.349|0.666|1.217|2.030

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse CD6 antibody|1
ST0000-10|Lyophilized recombinant mouse CD6 standard|10ng/tube�2
AR1107|Biotinylated anti- mouse CD6 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller Boster
Herstellerland USA
Sensitivity <10pg/ml
Specific Against Mouse
Antigen Name T-cell differentiation antigen CD6 (CD6)
Detection Range 62.5pg/ml-4000pg/ml
Alias CD_antigen, CD6, CD6 antigen Tp120, CD6_HUMAN, FLJ44171, OX52, T cell differentiation antigen CD6, T-cell differentiation antigen CD6, T12, TP120
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Datasheet