Mouse CD56/NCAM1 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse CD56 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

mouse CD56
Protein name: Neural cell adhesion molecule 1
Gene Full Name: neural cell adhesion molecule 1
abbreviation: NCAM1
Background: Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin(Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cellﾖcell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.
ID:P13595
ID:17967

alias

CD56 | NCAM 1 | NCAM C | NCAM1 | NCAM-1 | N-CAM-1 | P13591

Origin

Nativ and recombinant mouse CD56

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

NSO, L20-T711

sensitivity

<20pg/ml

detection range

625pg/ml-40000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|6.7|17.2|28.6|8.3|19.5|32.5
Standard deviation|0.281|0.946|1.83|3.984|1.326|2.34
CV(%)|4.2|5.5|6.4|4.8|6.8|7.2

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse CD56 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD56 has been precoated onto 96-well plates. Standards(NSO, L20-T711) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD56 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD56 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 40000pg/ml, 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml mouse CD56 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each mouse CD56 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|625|1250|2500|5000|10000|20000|40000 O.D.|0.040|0.081|0.128|0.229|0.440|0.772|1.400|2.259

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse CD56 antibody|1
ST0000-40|Lyophilized recombinant mouse CD56 standard|40ng/tube�2
AR1107|Biotinylated anti- mouse CD56 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4