Mouse CD30L ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse CD30L ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of CD30L in mouse serum, plasma and cell culture media. An antibody specific for mouse CD30L was coated on a 96-well plate. Standards and samples are added to the wells and any CD30L present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse CD30L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of CD30L present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Apoptosis;

specific against

Mouse

detection target

tumor necrosis factor (ligand) superfamily, member 8(CD30L)
Background: CD30 Ligand, also known as TNFSF8, is a type II membrane protein belonging to the TNF superfamily. CD30 Ligand is expressed on the surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.

alias

CD153, CD30LG, Cd30l, Tnlg3a

sensitivity

< 1 pg/ml

detection range

0.41-100 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P32972