Mouse CD26/DPP4 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse CD26 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Mouse CD26
Protein name: Dipeptidyl peptidase 4
Gene Full Name: dipeptidyl-peptidase 4
abbreviation: DPP4
Background: Dipeptidyl peptidase-4(DPP4), also known as adenosine deaminase complexing protein 2 or CD26(cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. By fluorescence in situ hybridization, the CD26 gene was mapped to 2q24.3. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. Furthermore, it appears to work as a suppressor in the development of cancer and tumours. CD26/ DPP4 plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others.
ID:P28843
ID:13482

alias

ADABP|ADCP-2|ADCP2|Adenosine deaminase complexing protein 2|CD26|Dipeptidyl peptidase 4|Dipeptidyl peptidase 4 soluble form|Dipeptidyl peptidase IV|Dipeptidyl peptidase IV membrane form|Dipeptidyl peptidase IV soluble form|Dipeptidylpeptidase 4|DPP 4|DPP IV|DPP4|DPP4_HUMAN|DPPIV|T-cell activation antigen CD26|TP103

Origin

Nativ and recombinant mouse CD26

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

NSO, S29-H760

sensitivity

<10pg/ml

detection range

62.5pg/ml-4000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|773|1540|2892|846|1685|3195
Standard deviation|39.4|104.72|216.9|54.99|124.69|274.77
CV(%)|5.1|6.8|7.5|6.5|7.4|8.6

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse CD26 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD26 has been precoated onto 96-well plates. Standards(NSO, S29-H760) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD26 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD26 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse CD26 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each mouse CD26 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.060|0.116|0.170|0.263|0.460|0.824|1.385|2.148

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse CD26 antibody|1
ST0000-10|Lyophilized recombinant mouse CD26 standard|10ng/tubex2
AR1107|Biotinylated anti- mouse CD26 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4