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Mouse CD23/FCER2 PicoKine ELISA Kit

Product Code: BOS-EK0924

Verfügbarkeit: Auf Lager

Mouse CD23/FCER2 PicoKine ELISA Kit

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Beschreibung

Details

Mouse CD23/FCER2 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse CD23 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

mouse CD23
Protein name: Low affinity immunoglobulin epsilon Fc receptor
Gene Full Name: Fc fragment of IgE, low affinity II, receptor for (CD23)
abbreviation: FCER2
Background: CD23, also known as Fc epsilon RII, or Fc?RII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the CD23 gene was assigned to 19p13.3. And CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.
UniProd ID:P20693
NCBI ID:14128

alias

BLAST 2|BLAST-2|C-type lectin domain family 4 member J|CD23|CD23 antigen|CD23A|CLEC4J|Fc fragment of IgE low affinity II receptor for (CD23)|Fc-epsilon-RII|FCE2|Fcer2|FCER2_HUMAN|IGEBF|Immunoglobulin E-binding factor|Low affinity immunoglobulin epsilon Fc receptor|Low affinity immunoglobulin epsilon Fc receptor soluble form|Lymphocyte IgE receptor

Origin

Nativ and recombinant mouse CD23

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

NSO, E50-P331

sensitivity

<15pg/ml

detection range

156pg/ml-10000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|485|1882|8430|597|2347|9105
Standard deviation|24.25|105.40|522.66|42.39|185.41|783.03
CV(%)|5.0|5.6|6.2|7.1|7.9|8.6

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse CD23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD23 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: E50-P331) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD23 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse CD23 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each mouse CD23 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.021|0.045|0.137|0.279|0.537|1.009|1.503|2.141

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse CD23 antibody|1
ST0000-10|Lyophilized recombinant mouse CD23 standard|10ng/tube�2
AR1107|Biotinylated anti- mouse CD23 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller Boster
Herstellerland USA
Sensitivity <15pg/ml
Specific Against Mouse
Antigen Name mouse CD23
Detection Range 156pg/ml-10000pg/ml
Alias BLAST 2, BLAST-2, C-type lectin domain family 4 member J, CD23, CD23 antigen, CD23A, CLEC4J, Fc fragment of IgE low affinity II receptor for (CD23), Fc-epsilon-RII, FCE2, Fcer2, FCER2_HUMAN, IGEBF, Immunoglobulin E-binding factor, Low affinity immunoglobu
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Datasheet