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Boster
For quantitative detection of mouse CD23 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Mouse
There is no detectable cross-reactivity with other relevant proteins.
mouse CD23Protein name: Low affinity immunoglobulin epsilon Fc receptorGene Full Name: Fc fragment of IgE, low affinity II, receptor for (CD23)abbreviation: FCER2Background: CD23, also known as Fc epsilon RII, or Fc?RII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the CD23 gene was assigned to 19p13.3. And CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor. ID:P20693 ID:14128
BLAST 2|BLAST-2|C-type lectin domain family 4 member J|CD23|CD23 antigen|CD23A|CLEC4J|Fc fragment of IgE low affinity II receptor for (CD23)|Fc-epsilon-RII|FCE2|Fcer2|FCER2_HUMAN|IGEBF|Immunoglobulin E-binding factor|Low affinity immunoglobulin epsilon Fc receptor|Low affinity immunoglobulin epsilon Fc receptor soluble form|Lymphocyte IgE receptor
Nativ and recombinant mouse CD23
a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
NSO, E50-P331
<15pg/ml
156pg/ml-10000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|485|1882|8430|597|2347|9105 Standard deviation|24.25|105.40|522.66|42.39|185.41|783.03 CV(%)|5.0|5.6|6.2|7.1|7.9|8.6
15-20
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s mouse CD23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD23 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: E50-P331) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD23 amount of sample captured in plate.
Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse CD23 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each mouse CD23 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.021|0.045|0.137|0.279|0.537|1.009|1.503|2.141
Catalog number|Description|Quantity-|96-well plate precoated with anti- mouse CD23 antibody|1ST0000-10|Lyophilized recombinant mouse CD23 standard|10ng/tube�2AR1107|Biotinylated anti- mouse CD23 antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30 mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <15pg/ml |
Specific Against | Mouse |
Antigen Name | mouse CD23 |
Detection Range | 156pg/ml-10000pg/ml |
Alias | BLAST 2, BLAST-2, C-type lectin domain family 4 member J, CD23, CD23 antigen, CD23A, CLEC4J, Fc fragment of IgE low affinity II receptor for (CD23), Fc-epsilon-RII, FCE2, Fcer2, FCER2_HUMAN, IGEBF, Immunoglobulin E-binding factor, Low affinity immunoglobu |