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Mouse CD22 PicoKine ELISA Kit

Product Code: BOS-EK1303

Verfügbarkeit: Auf Lager

Mouse CD22 PicoKine ELISA Kit

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Beschreibung

Details

Mouse CD22 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse CD22 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

mouse CD22
Protein name: B-cell receptor CD22
Gene Full Name: CD22 molecule
abbreviation: CD22
Background: CD22 or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. This gene is mapped to 19q13.2. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. Generally speaking, CD22 is a regulatory molecule that prevents the overactivation of the immune system and the development of autoimmune diseases. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signalling. This gene can downmodulate signaling through the IgM and IgD B-cell receptors(BCRs), but not through the IgG BCR, because the IgG cytoplasmic tail prevents CD22 phosphorylation and actually enhances IgG-BCR signaling.
UniProd ID:P35329
NCBI ID:12483

alias

B cell receptor CD22 precursor|B lymphocyte cell adhesion molecule|B-cell receptor CD22|B-lymphocyte cell adhesion molecule|BL CAM|BL-CAM|BLCAM|CD 22|CD22|CD22 antigen|CD22 molecule|CD22 protein|CD22_HUMAN|Lectin 2|Leu14|Lyb8|MGC130020|sialic acid binding Ig like lectin 2|Sialic acid binding immunoglobulin like lectin 2|Sialic acid-binding Ig-like lectin 2|SIGLEC 2|Siglec-2|SIGLEC2|T cell surface antigen Leu 14|T-cell surface antigen Leu-14

Origin

Nativ and recombinant mouse CD22

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

NSO, S22-R702

sensitivity

<10pg/ml

detection range

156pg/ml-10000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|1.86|4.32|7.35|2.54|5.13|7.44
Standard deviation|0.099|0.207|0.309|0.17|0.313|0.417
CV(%)|5.3|4.8|4.2|6.7|6.1|5.6

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse CD22 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD22 has been precoated onto 96-well plates. Standards(NSO, S22-R702) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD22 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD22 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse CD22 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each mouse CD22 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.039|0.080|0.116|0.221|0.394|0.701|1.150|1.992

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse CD22 antibody|1
ST0000-10|Lyophilized recombinant mouse CD22 standard|10ng/tube�2
AR1107|Biotinylated anti- mouse CD22 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller Boster
Herstellerland USA
Sensitivity <10pg/ml
Specific Against Mouse
Antigen Name B-cell receptor CD22 (CD22)
Detection Range 156pg/ml-10000pg/ml
Alias B cell receptor CD22 precursor, B lymphocyte cell adhesion molecule, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL CAM, BL-CAM, BLCAM, CD 22, CD22, CD22 antigen, CD22 molecule, CD22 protein, CD22_HUMAN, Lectin 2, Leu14, Lyb8, MGC130020, si
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Datasheet