The Mouse CD14 ELISA Pair Set is for the quantitative determination of Mouse CD14. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD14 coated on a 96-well plate. Standards and samples areadded to the wells, and any CD14present binds to theimmobilized antibody. The wells are washed and a horseradish peroxidaseconjugated rabbit anti-CD14 monoclonal antibody isthen added, producing an antibody-antigen-antibody "sandwich". The wells areagain washed and TMB substrate solution is loaded, which produces color inproportion to the amount of CD14present in thesample. To end the enzyme reaction, the stop solution is added and absorbancesof the microwell are read at 450 nm.
CD14Standard (antigene): Each vial contains 90 ng of recombinant mouse CD14. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 5 ng/mL is recommended. capture antibody type: 0.5 mg/mL of mouse anti-mouse CD14 monoclonal antibody. Dilute to a working concentration of 2 µg/mL in CBS before coating. (Catalog: # 50023-R217)detection antibody type: 0.5 mg/mL rabbit anti-mouse CD14 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 µg/mL in detection antibody dilution buffer before use. (Catalog: # 50023-R002)Lable: HRP
The minimum detectable dose of Mouse CD14 was determined to be approximately 78.1 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of Mouse CD14 was determined to be approximately 78.1 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
|Detection Range||78.1 pg/ml|