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Mouse CCL6 ELISA Kit

Product Code: BAS-3020311

Verfügbarkeit: Auf Lager

Mouse CCL6 ELISA Kit

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Beschreibung

Details

Mouse CCL6 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse CCL6 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of CCL6 in mouse serum, plasma and cell culture media. An antibody specific for mouse CCL6 was coated on a 96-well plate. Standards and samples are added to the wells and any CCL6 present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse CCL6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of CCL6 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Inflammation;

specific against

Mouse

detection target

chemokine (C-C motif) ligand 6(CCL6)
Background: Mouse C10, a member of the CC subfamily of chemokines, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GM-CSF. A human homolog for mouse C10 has not been identified. The expression of C10 mRNA has been detected in mouse monocytes and neutrophils, where it is strongly induced upon GM-CSF stimulation. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. 

alias

MRP-1, Scya6, c10

sensitivity

15 pg/ml

detection range

15.6-1000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P27784

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller BioAim Scientific
Herstellerland Kanada
Sensitivity 15 pg/ml
Specific Against Mouse
Antigen Name chemokine (C-C motif) ligand 6
Detection Range 15.6-1000 pg/ml
Alias MRP-1, Scya6, c10
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