BioAim Scientific (Kanada)
The Bioaim Mouse CCL6 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of CCL6 in mouse serum, plasma and cell culture media. An antibody specific for mouse CCL6 was coated on a 96-well plate. Standards and samples are added to the wells and any CCL6 present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse CCL6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of CCL6 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Inflammation;
chemokine (C-C motif) ligand 6(CCL6) Background: Mouse C10, a member of the CC subfamily of chemokines, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GM-CSF. A human homolog for mouse C10 has not been identified. The expression of C10 mRNA has been detected in mouse monocytes and neutrophils, where it is strongly induced upon GM-CSF stimulation. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF.
MRP-1, Scya6, c10
Serum, plasma, Cell culture supernatant
|Antigen Name||chemokine (C-C motif) ligand 6|
|Detection Range||15.6-1000 pg/ml|
|Alias||MRP-1, Scya6, c10|