BioAim Scientific (Kanada)
The Bioaim Mouse BMP-7 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of BMP-7 in mouse serum, plasma and cell culture media. An antibody specific for mouse BMP-7 was coated on a 96-well plate. Standards and samples are added to the wells and any BMP-7 present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse BMP-7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of BMP-7 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Embryogenesis and morphogenesis;
bone morphogenetic protein 7(BMP-7) Background: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene. The protein encoded by this gene is a member of the TGF-β superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder. BMP7 induces the phosphorylation of SMAD1 and SMAD5, which in turn induce transcription of numerous osteogenic genes. It has been demonstrated that BMP7 treatment is sufficient to induce all of the genetic markers of osteoblast differentiation in many cell types.
Serum, plasma, Cell culture supernatant
|Antigen Name||bone morphogenetic protein 7|
|Detection Range||4.1-1000 pg/ml|