Mouse BAFF R ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse BAFF R ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of BAFF R in mouse serum, plasma and cell culture media. An antibody specific for mouse BAFF R was coated on a 96-well plate. Standards and samples are added to the wells and any BAFF R present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse BAFF R antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of BAFF R present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Apoptosis;

specific against

Mouse

detection target

tumor necrosis factor receptor superfamily, member 13c( BAFF R )
Background: BAFF receptor (B-cell activating factor receptor, BAFF-R), also known as tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), is a membrane protein of the TNF receptor superfamily which recognizes BAFF. In humans it is encoded by the TNFRSF13C gene. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival. Overexpression of BAFF in mice results in mature B-cell hyperplasia and symptoms of systemic lupus erythematosus (SLE). Also, some SLE patients have increased levels of BAFF in serum. Therefore, it has been proposed that abnormally high levels of BAFF may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells.

alias

2010006P15Rik, BAFF-R, Baffr, Bcmd, Bcmd-1, Bcmd1, Lvis22

sensitivity

15 pg/ml

detection range

28.7-7000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q9D8D0