Interleukin-8 Kit

Brand

GENWAY

Overview

Intended Use: Immunoenzymetric assay for the in vitro quantitative measurement of human interleukin-8 (IL-8) in plasma. Background: A. Biological activitiesIL-8 (also known as NAP-1 for Neutrophil-activating peptide) is a chemoattractant protein for neutrophils. This cytokine belongs to a new family of chemotactic peptides called \"chemokines\". This proin¬ammatory mediator is secreted by di erent cells such as monocytes neutrophils endothelial cells broblast after activation and by mitogen-stimulated T lymphocytes. The roleof IL-8 in the recruitment of neutrophils in the lung during ARDS has also been suggested. Principles of the method: The Genway IL-8-EASIA is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on microtiterplate. The assay uses monoclonal antibodies (MAbs) directed against distinct epitopes of IL-8. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1 - human IL-8 - MAb 2 - HRP the microtiterplate is washed to remove unbound enzyme labelled antibody. Bound enzymelabelled antibody is measured through a chromogenic reaction. Chromogenic solution (TMB) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance which is proportional to the IL-8 concentration. A calibration curve is plotted and IL-8 concentration in samples is determined by interpolation from the calibration curve. The use of the EASIA reader (linearity up to 3 OD units) and a sophisticated data reduction method (polychromatic data reduction) result in a high sensitivity in the low range and in an extended calibration range. Storage and Stability: ? Before opening or reconstitution all kits components are stable until the expiry date indicated on the vial label if kept at 2 to 8C. ? Unused strips must be stored at 2-8C in a sealed bag containing a desiccant until expiration date. ? After reconstitution calibrators controls and Specimen Diluent are stable for 4 days at 2 to 8C. For longer storage periods aliquots should be made and kept at -20C for maximum 2 months. Avoid successive freeze thaw cycles. ? The concentrated Wash Solution is stable at room temperature until expiration date. ? Freshly prepared Working Wash solution should be used on the same day. ? After its first use the conjugate is stable until expiry date if kept in the original well-closed vial at 2 to 8C. ? The freshly prepared revelation solution is stable before use for maximum 15 minutes at room temperature and must be discarded afterwards. ? Alterations in physical appearance of kit reagents may indicate instability or deterioration. Sensitivity and Specificity: No significant cross-reaction was observed in presence of 50 ng of IL-1? IL-1? IL-1ra IL-2 IL-3 IL-4 IL-6 IL-7 IL-10 TNF-? TNF-? IFN-? IFN-? IFN-? TGF-? GM-CSF OSM MIP-1? MIP-1? LIF MCP-1 G-CSF RANTES PF-4 ?TG GRO IP-10 and SCF. This IL-8 assay is specific for human natural and recombinant IL-8 and is able to recognize the 72 a. a. form of IL-8. Product Name: Interleukin-8 Function: IL-8 is a chemotactic factor that attracts neutrophils basophils and T-cells but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77) respectively. Subunit: Homodimer. Subcellular Location: Secreted. Ptm: Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes leukcocytes and endothelial cells. In general IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. Similarity: Belongs to the intercrine alpha (chemokine CxC) family.

spezie

other

detection target

Interleukin-8

pack size

96 tests