The influenza B HA ELISA Pair Set is for the quantitative determination of influenza B HA This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Influenza B HA coated on a 96-well plate. Standardsand samples are added to the wells, and any Influenza B HA presentbinds to the immobilized antibody. The wells are washed and a horseradishperoxidase conjugated rabbit anti-Influenza B HApolyclonal antibody is then added, producing an antibody-antigen-antibody"sandwich". The wells are again washed and TMB substrate solution is loaded,which produces color in proportion to the amount of Influenza B HA present in the sample. To end theenzyme reaction, the stop solution is added and absorbances of the microwellare read at 450 nm.
HAStandard (antigene): Each vial contains 145 ng of recombinant influenza B HA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4 ng/mL is recommended. capture antibody type: 0.5 mg/mL of mouse anti-influenza B HA monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 µg/mL in PBS before coating. (Catalog: # 11053-MM09)detection antibody type: 0.2 mg/mL rabbit anti-influenza B HA polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4). Dilute to working concentration of 0.5 µg/mL in detection antibody dilution buffer before use.Lable: HRP
The minimum detectable dose of Influenza B HA was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of Influenza B HA was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard|
|Antigen Name||Influenza B Hemagglutinin|
|Detection Range||62.5 pg/ml|
|Alias||Influenza B Hemagglutinin / HA|