The H5N1 (avian flu) hemagglutinin ELISA Pair Set is for the quantitative determination of hemagglutinin. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for hemagglutinin coated on a 96-well plate. Standards and samples are added to the wells, and any hemagglutinin present binds to the immobilized antibody. The wells are washed and a biotinylated rabbit anti-hemagglutinin polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". To produces color in proportion to the amount of hemagglutinin present in the sample strepavidin-HRP and TMB substrate solution are loaded. The absorbances of the microwell are read at 450 nm.
This H5N1 HA / Hemagglutinin ELISA Pair Set (SEK002) can quantificationally detect Anhui-HA (A/Anhui/1/2005), Indonesia-HA (A/Indonesia/5/2005), and Vietnam-HA (A/Vietnam/1194/2004). And it can also react with Turkey-HA (A/turkey/Turkey/1/2005) and Qinghai-HA (A/bar-headed goose/Qinghai/14/2008).
HAStandard (antigene): Each vial contains 150 ng of recombinant H5N1 hemagglutinin. Reconstitute with 1 mL detection antibody dilution buffer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 5000 pg/mL is recommended. capture antibody type: 0.5 mg/mL of mouse anti-H5N1 hemagglutinin monoclonal antibody. Dilute to a working concentration of 0.5 µg/mL in PBS before coating. (Catalog: # 11048-MM14)detection antibody type: 0.5 mg/mL biotinylated rabbit anti-H5N1 hemagglutinin polyclonal antibody. Dilute to a working concentration of 1.5 µg/mL in detection antibody dilution buffer before use.Lable: Biotin
The minimum detectable dose of H5N1 hemagglutinin (HA) was determined to be approximately 78.125 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles. Streptavidin-HRP: Store at 4°C and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.
|Sensitivity||The minimum detectable dose of H5N1 hemagglutinin (HA) was determined to be approximately 78.125 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
|Antigen Name||Influenza A H5N1 (Avian Flu) Hemagglutinin|
|Detection Range||78.125 pg/ml|
|Alias||Influenza A H5N1 (Avian Flu) Hemagglutinin / HA|