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Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set

Product Code: SEK001

Verfügbarkeit: Auf Lager

Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set

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Details

Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set

Brand

Sino Biological

short description

The Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set is for the quantitative determination of Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA coated on a 96-well plate. Standards and samples are added to the wells, and any Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

spezie

Influenza

Cross Reaction

The following Hemagglutinin of different influenza virus types and subtypes prepared at 200 ng/mL were tested and No cross-reactivity was identified: H1N1 (A/Brisbane/59/2007) H5N1 (A/Anhui/1/2005) H5N1 (A/Vietnam/1194/2004) H3N2 (A/Brisbane/10/2007) Influenza B (B/Florida/4/2006)

detection target

HA
Standard (antigene): Each vial contains 100 ng of recombinant Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
capture antibody type: 0.5 mg/mL of mouse anti-Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 µg/mL in PBS before coating. (Catalog: # 11055-MM04)
detection antibody type: 0.2 mg/mL of rabbit anti-Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4°C;). Dilute to working concentration of 1 µg/mL in detection antibody dilution buffer before use.
Lable: HRP

sensitivity

The minimum detectable dose of Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA was determined to be approximately 15.625 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Detection limitation

15.625 pg/ml

certificate

ISO9001-2008

pack size

5 and 15 Plates

storage and Stability

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.

Weitere Informationen

Weitere Informationen

Hersteller Sino Biological
Herstellerland China
Sensitivity The minimum detectable dose of Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA was determined to be approximately 15.625 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicat
Specific Against Influenza
Antigen Name Influenza A H1N1 (Swine Flu 2009) Hemagglutinin
Detection Range 15.625 pg/ml
Zertifikat ISO9001-2008
Alias Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA
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