HumanFasL ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human FasL ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of FasL in human serum and plasma. An antibody specific for human FasL was coated on a 96-well plate. Standards and samples are added to the wells and any FasL present binds to the immobilized antibody. The wells are washed and biotinylated anti-human FasL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of FasL present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Apoptosis;

specific against

Human

detection target

Fas ligand(FasL )
Background: Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a transmembrane protein homotrimer that binds to Fas/CD95 and triggers apoptosis in the Fas-expressing cell. Fas Ligand also binds the soluble decoy receptor DcR3. It is expressed on activated Th1 cells, CD8+ cytotoxic T cells, and NK cells. A soluble form can be released which remains trimeric and retains the ability to bind Fas. Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintenance of immune privileged sites. Tumor cells can evade immune surveillance by up-regulating Fas Ligand to kill tumor infiltrating lymphocytes. In mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity.

alias

ALPS1B, APT1LG1, APTL, CD178, CD95-L, CD95L,FASL, TNFSF6, TNLG1A

sensitivity

< 1 pg/ml

detection range

1.4-1000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P48023