For the quantitative determination of Human VSIG4 concentration in cell lysate.
The concentration of Human VSIG4 detected in a Hela cell lysate is 6.3ng/mL.The Hela cell lysate' total protein concentration was about 9.3mg/mL.
This assay recognizes both recombinant and natural Human VSIG4. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
VSIG4Antigene: 12163-H08Hcapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||39.1-2000 pg/ml|