The human VEGFR3 / FLT4 ELISA Pair Set is for the quantitative determination of human VEGFR3 / FLT4. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for VEGFR3 /FLT4 coated on a 96-well plate. Standards and samples are added to the wells,and any VEGFR3 / FLT4 present binds to the immobilized antibody. The wells arewashed and a horseradish peroxidase conjugated mouse anti-VEGFR3 / FLT4monoclonal antibody is then added, producing an antibody-antigen-antibody"sandwich". The wells are again washed and TMB substrate solution is loaded,which produces color in proportion to the amount of VEGFR3 / FLT4 present inthe sample. To end the enzyme reaction, the stop solution is added andabsorbances of the microwell are read at 450 nm.
FLT4Standard (antigene): Each vial contains 80 ng of recombinant VEGFR3. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A six-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 5 ng/mL is recommended capture antibody type: 0.5 mg/mL of mouse anti-VEGFR3 monoclonal antibody. Dilute to a working concentration of 2 µg/mL in CBS before coating (Catalog: # 10806-MM02)detection antibody type: 0.4 mg/mL mouse anti-VEGFR3 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 µg/mL in detection antibody dilution buffer before use (Catalog: # 10806-MM06)Lable: HRP
The minimum detectable dose of human VEGFR3 / FLT4 was determined to be approximately 156.1 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of human VEGFR3 / FLT4 was determined to be approximately 156.1 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
|Detection Range||156.1 pg/ml|
|Alias||VEGFR3 / FLT4|