Human VEGF R2 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human VEGF R2 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of VEGF R2 in Human serum, plasma and cell culture media. An antibody specific for Human VEGF R2 was coated on a 96-well plate. Standards and samples are added to the wells and any VEGF R2 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF R2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of VEGF R2 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; Cancer Biomarker;

specific against

Human

detection target

kinase insert domain receptor (VEGF R2)
Background: VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis. Kinase insert domain receptor has been shown to interact with SHC2, Annexin A5 and SHC1.

alias

CD309, FLK1, VEGFR,VEGFR2

sensitivity

30 pg/ml

detection range

34.3-25000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P35968